FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_O2_Strep201907_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_O2_Strep201907_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14404289
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250210.17370520683110427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG73800.011.691031145
AGTCACC76850.010.849662145
CCAGTCC76700.09.452938145
GCTTGAA692150.08.401105145
CTGAACA108200.08.242142145
CGCCAAA113850.07.769428145
CTTGAAA727300.07.765798145
TCTGCTT852400.07.2725196145
AAAAAAA2356850.07.155501145
TGCTTGA822450.07.096572145
GAAAAAA739950.07.0941186145
TCTTCTG1008300.06.9318557145
CAATATG91850.06.867558145
CTGCTTG821250.06.8597436145
TTCTGCT915900.06.8474727145
AGGGGGG348950.06.835888145
CCGTCTT1080350.06.805119145
GAGCACC137750.06.631949145
GCCGTCT1140500.06.350858145
TGAAAAA731100.06.3072867145
Produced by FastQC (version 0.11.5)