FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_KN1_Strep201907_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_KN1_Strep201907_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506885
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG902650.7844434006249302No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213070.18516740195109277No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176320.15323000099505643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG133350.024.376531
ATGCCGG36250.015.401054145
ACCAATA111250.010.558027145
CCAATCG25400.010.276295145
CCGTAGT81600.09.5949117
GTAGCCG86100.09.5188533
GCCGTAG91150.08.9119086
AGCCGTA93300.08.8620175
TCACTGC98200.08.417073145
GTAAACT88950.08.4019121
AGGGGGG349800.08.4008271
GCTTGAA468900.08.133421145
TGTAGCC101950.08.1111012
CACACGG19102.1209416E-97.97175145
GTTACGA108900.07.7955151
GATCCGT78150.07.70612341
GTGTAGC107550.07.69097331
CTGAACA105700.07.4082656145
AAAGGGG287450.07.3904867145
CGTCTTA11953.7943712E-47.2808332145
Produced by FastQC (version 0.11.5)