FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_KN1_Strep201907_ChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_KN1_Strep201907_ChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16834689
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC188930.11222660543357824TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATG91550.012.988447145
ATGCCGG62700.012.604646145
GTATGCC536200.07.3830795145
CCGTCTT426500.06.5790563145
GAGCACC95400.06.4601445145
CTCGTAT598450.06.17894145
TCGTATG591750.06.175384145
CGTATGC540150.06.1559267140-144
GCTTGAA298150.06.1282635145
GCCGTCT506850.06.065381145
TCTCGTT56100.05.815959145
TCTCGTA603500.05.8028526145
ATCTCGT664950.05.779078145
CTTGAAA332500.05.7568374145
TATGCCG540650.05.7264223145
CATCTCG722050.05.633357145
TAGGCAT828800.05.493911145
TTAGGCA907700.05.4157557145
ACTTAGG961400.05.3922944145
TGCCGTC542150.05.349488145
Produced by FastQC (version 0.11.5)