FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_KN1_Strep201906_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_KN1_Strep201906_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8661631
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAATG14950.012.124861145
CCAGTCC9151.2611963E-711.093917145
GAGCACC13954.7293724E-1110.395221145
CGTATGC81600.08.796764145
GGAAGAC14851.5914338E-88.788687145
CCAACTA165350.08.638532145
CCGTCTT61400.08.620499145
GTATGCC80400.08.477147145
ATGCCGG7700.0031490498.474805145
GCTTGAA38700.08.243651145
CTAATCT121650.08.165568145
ACACGTA8900.00127540398.146816145
AGTCACC216550.07.968869145
TCACCAA211200.07.8960857145
AACTAAT153800.07.8258176145
ACCAACA19954.940375E-97.6322803145
CTTGAAA46750.07.2894406145
TCTCGTA95700.07.197673140-144
TCGTATG114350.07.1650662145
GTCACCA226500.07.0746017145
Produced by FastQC (version 0.11.5)