FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_GFP_Strep201907_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_GFP_Strep201907_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10120238
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1006510.994551709159409No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195640.19331561174747075No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149680.14790166002024852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG33000.025.266237145
TGGGGGG124250.023.065351
ATGCCGG41050.022.254309145
TCTCGTT49750.013.261877145
CGTCTTA12800.012.461474145
GCTTGAA270700.08.731442145
AGGGGGG263100.08.2729381
ACGCTGT77000.07.34683135
GTCACCC53700.07.2908163145
AAGAGCC48550.07.168176145
CCGTAGT74800.07.07527977
TGAAAAA342150.06.8868957145
CGCTGTA79200.06.8680316
GTAGCCG82400.06.77734663
CTTGAAA316700.06.661954145
CCGTCTT458700.06.6228046145
TCTTCTG438050.06.6205335145
TTCTACG72300.06.6180197
TCTGCTT357300.06.594882145
GCTGTAG89150.06.50565437
Produced by FastQC (version 0.11.5)