FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_GFP_Strep201906_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_GFP_Strep201906_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10612879
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA11502.6277121E-810.087892145
CGTATGC106450.09.535877145
CACACGG7903.897065E-49.178067145
CCGTCTT77850.08.475416145
AGCCCGC9753.471487E-48.180246145
GTATGCC104700.08.033219145
GTACATA12751.1514032E-57.961523145
TCTCGTA123250.07.9419127145
AGTCACC256450.07.5772285145
TCTACCT19151.8568244E-87.57507372
GATCGGC25552.910383E-117.3770549
TCTGAAC424500.07.3275347145
ATCTCGT132800.06.737479140-144
CCAAAAG216600.06.6949883145
ACCTTCT28253.1286618E-106.67444373
CACACGT558800.06.6693726145
AAGATCT163050.06.5814137145
CTCCAGT357600.06.549125145
CGTCTGA463800.06.42524145
AGCACAC624000.06.4140563145
Produced by FastQC (version 0.11.5)