FastQCFastQC Report
Thu 18 Jul 2019
HMWG7AFXY_n01_GFP_Strep201906_ChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWG7AFXY_n01_GFP_Strep201906_ChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8019139
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC39700.08.401331145
CGGTAGC79900.07.89502145
CATCTCG68000.07.677227145
ACACGTC225150.07.4713106145
CACACGT237950.07.404596145
AGTCACG115450.07.34806145
CGTCTGA193550.07.342495145
CACGGTA80000.07.2507143145
TCTGAAC179600.07.226491145
GTAGCAT75400.07.2122483145
GTCTGAA217550.07.032409145
CTCCAGT159950.07.0263247145
ACGGTAG75850.06.978275145
GTCACGG116800.06.6423492145
GCACACG254200.06.5319176145
AGAGCAC307850.06.5241117145
CTCGTAT62400.06.5070505145
ATCGGAA405700.06.505447145
AGCACAC278450.06.483849145
CACGTCT283800.06.3360715145