FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15987489
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10525786.583760589295792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTT5850.023.9406810
CACTCTT10250.020.1911752
TCCCTAC5000.018.9082789
TTCCCTA5700.018.4309128
CGGTTCG4700.017.87887812
CCGTATC4350.016.0868549
GCCGTAT4800.016.03577448
CGCCGTA4500.015.55110747
GTTCGGC5600.014.38526314
CCGATCT6450.014.11320141
TGAACCG6550.013.887156529
CGTATCA5300.013.862699550
TAGACCG2556.4159703E-613.7370565
TCCGATC6500.013.46494440
CGACGCT5750.013.39717
CCCTTAG3507.1340764E-813.0369141
TATACTG11400.012.9056025
TTTCCCT9400.012.6671447
CTATACT7300.012.4802894
TAGGACA5651.8189894E-1212.4038274
Produced by FastQC (version 0.11.5)