FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n02_LB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n02_LB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67042904
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGAG1436500.013.40321870
CGAATAG1725450.013.234179570
CCCTTAG117000.013.2271591
GTATAGG172400.012.8354161
TATACAG515500.012.60935
TATACTG409900.012.4352795
TAGACCG92500.012.4052655
GAATAGA1595750.012.38591470
GTATATA785250.012.1503481
GTATAGA308600.012.0378691
CTTAGAC180400.011.8701053
GTATTAG239250.011.7661461
CTATACT231450.011.7303914
TACACTG429500.011.5908595
ACTGTGC279900.011.560548
GTATAAG273200.011.3548961
TTATACT424150.011.26784
TCTATAC310450.011.2480843
GTATTAT511250.011.1356951
TATACCG136150.011.1261325
Produced by FastQC (version 0.11.5)