FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n02_LB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n02_LB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50857476
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG582950.11462424914677244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG93800.013.2513241
TAGACCG77150.013.1047225
TATACTG327550.013.0514725
CTTAGAC141950.012.7681313
GTATTAG188450.012.5412661
TTATACT334150.012.3966864
TATACAG431700.012.3824675
CGAATAG1156750.012.20843670
GTATATA669150.012.1760581
GTATAGA256850.012.0232751
AATAGAG1043100.011.89745270
GTATAGG142650.011.5361221
GTATTAT399650.011.3805471
CTATACT184000.011.3514634
TATACCG109650.011.3262355
GAATAGA1106850.011.18778669
TAATACT301150.011.1760314
GTATAAT364750.011.1159621
GTATAAG217350.011.1153521
GTGTAGG198100.010.9582441
Produced by FastQC (version 0.11.5)