FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n02_Bay2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n02_Bay2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50440457
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG528580.10479286498137795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGAG1460050.013.65246770
TAGACCG68850.012.499545
TAGAGAG918350.012.15266470
GAATAGA1636800.012.1425869
CCCTTAG95200.012.1372131
CGAATAG1793800.011.988584570
TATACAG374000.011.8607055
CTTAGAC138850.011.7428493
TATACTG303500.011.5382055
ATAGAGA1238350.011.5085170
CTATACT170350.011.3162374
GTATATA505300.011.135471
GGTGTAG367200.011.10805768
TAATACT271000.010.8185074
TTATACT283700.010.7041694
GTATTAG173000.010.6458821
GTATTAA265750.010.5931421
GTGTTAG233600.010.2374051
GTATTAT346050.010.1485521
GATCTCG135100.010.13171270
Produced by FastQC (version 0.11.5)