FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n02_Bay1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n02_Bay1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58791694
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG138650.017.4491421
TAGACCG88550.015.3680775
CTTAGAC198100.014.8183153
CCTTAGA170500.014.3477162
TTATACT293450.013.2097224
TATACTG333450.013.19800955
GTATTAG188750.012.8732571
TATACAG438650.012.6805325
GTATATA522100.012.6139471
CTATACT190750.012.6002814
AATAGAG1664550.012.25704370
GTATAGA270750.012.2072941
GGTGTAG525000.012.11598370
TAATACT261150.012.0838284
CGAATAG1948700.012.0039670
TATACCG118850.011.9210455
ATCTCGG133700.011.83590669
GTATAAT299050.011.6491761
TCTATAC261800.011.6137223
GTATAAG227050.011.5344271
Produced by FastQC (version 0.11.5)