FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15987489
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8566945.358527533623322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27900.032.5322951
GGCTCGG5800.019.9390771
GCTCGGA5650.019.2043972
TCGGAGA5850.019.1411844
GTCCTAG3900.016.1743561
CGGAGAT7000.014.9969165
GTGTTAG11050.014.9057811
GTATATA27250.014.4035871
CTCGGAG7600.014.2746753
CGGGGGG66650.013.1449531
CCCTTAG5255.456968E-1212.6827511
GTAGGAC5003.092282E-1112.5985533
GTATAGA12100.012.4538111
TACCCCG4509.858923E-1012.4418865
GTATAGG6800.012.3686271
TGAACCG7100.012.31809613
ACCGATC6350.012.12971533
TAGACTG8950.012.1204135
TAGTACT8750.011.997424
AGGACAG9050.011.98648555
Produced by FastQC (version 0.11.5)