FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n01_LB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n01_LB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67042904
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCA1277700.015.04975770
TAGACCG96350.014.2383515
GACAGGC1486350.013.30923170
TATACTG416000.013.2162485
TATACAG519200.013.1102285
CCCTTAG116350.012.8159981
CAGGCAG974500.012.75968370
GTATATA774600.012.70261
CTATACT230100.012.6538854
CTTAGAC179100.012.544523
TTATACT440450.012.537984
AATCTCG279550.012.50989869
AGACAGG1879900.012.39817670
ATCTCGT289000.012.30678670
GTATAGA319050.012.2876421
TATACCG133150.012.1692875
TAATACT391050.011.9561514
GTATAAG265700.011.8829491
TCTATAC306300.011.8366123
GTATAGG170750.011.8078561
Produced by FastQC (version 0.11.5)