FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n01_LB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n01_LB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50857476
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC793250.015.06176770
CTTAGAC142750.013.6549323
TATACTG336450.013.4804615
CCGTACT602950.013.43481670
CTATACT186950.013.346724
TAGACCG77400.013.2478975
GACCGTA935650.012.87796270
CGTACTA477700.012.86090770
CCCTTAG96800.012.4752551
TATACAG430300.012.3783745
GTATAGG146650.012.3638411
GATCTCG215550.012.27794369
GTATTAG184700.012.166731
GTATAGA251700.012.1544111
AGACCGT1166350.012.13157370
GTATATA623750.012.1269031
TTATACT343750.012.0231334
TAATACT305350.011.9535484
ATCTCGT223900.011.94507770
GTATAAG219350.011.6650181
Produced by FastQC (version 0.11.5)