FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n01_Bay2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n01_Bay2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50440457
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT252250.014.57133370
ACTCCTG1371550.014.4638170
TCCTGAG749500.014.3526570
CTCCTGA985500.014.32914970
CATCTCG258300.014.24363369
CCCTTAG97400.013.9077821
TAGACCG69400.013.0102745
GACTCCT1528750.012.9650670
CTTAGAC141600.012.3079033
AGACTCC1833350.011.8344370
GTATATA499150.011.5566491
CTATACT166850.011.49404054
TATACAG377050.011.2494125
TATACTG307950.011.1257335
GCATCTC332350.011.07045468
GTATTAG168200.010.9046341
GTATAGA228400.010.8043561
TTATACT295700.010.6988124
CTCGTAT169500.010.69796770
GTATTAT327300.010.5447611
Produced by FastQC (version 0.11.5)