FastQCFastQC Report
Sat 15 Sep 2018
HMWC2BGX7_n01_Bay1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMWC2BGX7_n01_Bay1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58791694
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG131550.017.5346871
TAGACCG89300.016.8514085
TATCTCG345900.016.47595269
ATCTCGT349050.016.39752470
CTTAGAC197800.015.4629843
ACGGACT1357000.014.64234770
CCTTAGA170350.014.4421932
TATACTG329700.013.225695
CTATACT180900.012.8258034
TATACAG431600.012.8032425
GTATAGA258850.012.6299661
GTATTAG185300.012.4672881
GTATATA461900.012.4582581
GTATAGG160800.012.4512641
GGACTCC897450.012.2012670
CCTATCT502950.012.20123569
TTATACT291350.012.1675764
CTATCTC513300.012.11893270
GTATAAG216650.011.9718971
GACGGAC1682150.011.95353669
Produced by FastQC (version 0.11.5)