FastQCFastQC Report
Thu 19 Oct 2017
HMVKTBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKTBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370054328
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24914360.6732622243510148No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT8817340.23827150050248838No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC4177290.11288315482152664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT2911100.021.8398556
GTTATAC3032250.020.4690323
TATACCG3098100.020.3343895
TGGACCA3357350.020.1986275
TACCGGT3059950.019.846667
ATACCGG3216200.019.5793466
TTATACC3146250.019.5643864
GTACTGG2632600.019.1190971
GGGGTAT2952050.019.10691522
GGTATTC2955100.019.02948824
CATGTGG3363150.018.93155510
CCATGTG3435400.018.8979479
TGTTATA3388250.018.891482
GGGTATT3004150.018.7847423
GACCATG3529450.018.615867
TCAGGGG3184450.018.49203318
TGGCATA2919350.018.22002436
TTCAGGG3246450.018.15830617
GTGTTAT3515650.018.1469121
ATTGGAC1185350.018.0855313
Produced by FastQC (version 0.11.2)