FastQCFastQC Report
Thu 19 Oct 2017
HMVKTBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKTBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370054328
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAA7937950.013.9319391
CCTTCTT6442950.013.9002711
CCTCTCT6269300.013.8962694
ACACCTT5310900.013.8788162
TACACCT5307800.013.8734491
TCAACCG4851100.013.8651331
CTCCTAC5041300.013.863594
ACCTCTC6252400.013.8530513
TACAAGA7968600.013.8495653
AGACATC8072750.013.8450462
AACCGAT4890850.013.8432543
CACCTTC5315950.013.8375513
ACATCGC8043600.013.8372554
TCCTACG5047200.013.8331245
CCTCAGT6010400.013.8308216
CCTACGT5041700.013.8286966
CCACACT3506450.013.825852
GACCTCT6268550.013.8236842
TCTCCTA5063250.013.8234563
CCTTCGT5312900.013.8200335
Produced by FastQC (version 0.11.2)