FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n02_rnaseq_dmso2_10_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n02_rnaseq_dmso2_10_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16503594
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG644980.3908118437717263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA24950.024.47428147
TCCGATC84900.022.4720026
AGAGTAC89550.020.91820118
AGTACGG68450.020.8648720
CCGATCT94650.020.375847
GAGTACG70600.020.22952819
TACGGGG45800.020.11104422
GCCGTAT31400.019.99675448
GTACGGG71750.019.9533121
CGCAGAG97050.019.44353915
CTTCCGA99200.019.4094834
CGATCTA32100.018.4847478
TCTTCCG112950.017.2645423
ACGCAGA111350.017.07044614
AGTGGTA113700.016.9645674
CAGAGTA115650.016.40616817
CCGTATC39800.016.12296749
AACGCAG118200.016.08112113
GTGGTAT119950.016.0495245
GTAGGGA39600.016.02923419
Produced by FastQC (version 0.11.2)