FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n02_rnaseq_dmso1_10_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n02_rnaseq_dmso1_10_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14768828
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG482530.3267219308126549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC82250.023.3210096
GAGTACG57500.021.35846519
AGTACGG59250.020.90229420
CTTCCGA93400.020.7997574
CCGATCT93050.020.7254477
AGAGTAC74700.020.64323418
CGATCTA31550.020.3376838
GTACGGG60650.020.24916521
TACGGGG39050.019.61197522
CGCAGAG81400.019.0284115
CGCCGTA17500.018.33340547
TCTTCCG108100.018.0076083
TTCCGAT112350.017.2882545
ACGCAGA93700.016.71457514
CAGAGTA96900.016.1630917
GCTCTTC124600.016.1417561
GTGGTAT98200.015.8445855
AGTGGTA98450.015.8423444
AACGCAG100300.015.51158413
TCAACGC104400.014.73714711
Produced by FastQC (version 0.11.2)