FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n02_rnaseq_dmso1_10_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n02_rnaseq_dmso1_10_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15343336
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG658590.42923520673730925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA21700.025.75479347
TCCGATC96000.022.8201246
CCGATCT105450.020.8731587
GCCGTAT27600.020.74996648
CTTCCGA109000.020.5455254
GAGTACG57300.019.62689419
CGATCTA38200.019.145198
AGTACGG60300.018.47880220
TCTTCCG123550.018.1583273
AGAGTAC72900.018.12406518
TACGGGG39300.017.90713922
GTACGGG63400.017.73850321
TTCCGAT131250.016.9803755
CCGTATC34150.016.77010349
CGTATCA35350.016.10338450
CGCAGAG83950.015.90247515
GCTCTTC142250.015.7886061
TCGCCGT36900.015.61318446
GGTCGCC37150.015.41580544
GTAGGGA38100.015.3926219
Produced by FastQC (version 0.11.2)