FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n02_rnaseq_bpa2_10_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n02_rnaseq_bpa2_10_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14702536
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG591910.4025904102530339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG65400.027.85145619
AGAGTAC86900.027.7095118
AGTACGG68750.026.44415320
CGCAGAG95450.025.44383215
GTACGGG72500.025.17151821
TACGGGG48550.024.869722
TCCGATC75000.024.2872946
ACGCAGA106850.022.66446514
AGTGGTA107650.022.6608094
CAGAGTA110200.022.03891417
GTGGTAT112700.021.9791775
AACGCAG112600.021.6909813
TCAACGC114200.021.32651911
CCGATCT85800.021.1497387
GCAGTGG117000.020.9806212
CTTCCGA91850.020.0600554
AGCAGTG122650.020.0592771
GCAGAGT123450.020.03674516
TGGTATC127000.019.3411716
CGATCTA29550.018.6782638
Produced by FastQC (version 0.11.2)