FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n02_rnaseq_bpa2_10_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n02_rnaseq_bpa2_10_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14590793
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG506050.3468283046713088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG57000.023.8458119
AGTACGG57550.023.55789220
AGAGTAC76400.022.75775318
TACGGGG40150.022.59760522
GTACGGG62550.021.84022321
TCCGATC61300.021.610856
CGCAGAG83550.021.05772415
ACGCAGA93900.018.77346814
AGTGGTA96650.018.4215584
CCGATCT71850.018.149567
AACGCAG101350.017.56372613
CAGAGTA100750.017.49723817
GTGGTAT103400.017.2495215
CTTCCGA77900.017.185924
TCAACGC105200.016.98647311
AGCAGTG108750.016.7762721
GCAGTGG108600.016.6902432
CGATCTA26150.015.9623548
TCTTCCG87550.015.3740473
TGGTATC117700.015.2121996
Produced by FastQC (version 0.11.2)