FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n02_rnaseq_bpa1_10_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n02_rnaseq_bpa1_10_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17622163
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG542200.30768073136084373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG46900.022.43486422
AGTACGG69600.022.4039420
TCCGATC107500.022.3046916
GAGTACG71050.021.99520919
GTACGGG72900.021.29512621
AGAGTAC90200.020.99712218
CCGATCT117750.020.2458957
CTTCCGA120800.020.0521774
CGCAGAG97750.019.5515915
CGATCTA42350.018.4908378
TCTTCCG134050.018.1768763
ACGCAGA111800.017.46480214
TTCCGAT147450.016.4487955
AGTGGTA118700.016.3081044
CAGAGTA119450.016.05785617
AACGCAG120950.015.94391413
GTGGTAT124050.015.8804535
TCAACGC121100.015.81021911
GCTCTTC157150.015.5677461
GCAGTGG131400.014.7400182
Produced by FastQC (version 0.11.2)