FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n01_rnaseq_dmso2_10_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n01_rnaseq_dmso2_10_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16503594
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172760.10468022904586721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27700.022.41704649
TCCGATC83700.022.016136
GAGTACG70600.021.74414319
AGTACGG71700.021.21809620
AGAGTAC116850.020.84294118
TACGGGG48800.020.6412322
GTACGGG75500.020.14949421
CGCAGAG122750.019.95365715
CCGATCT93550.019.83897
CTTCCGA99400.018.9899924
AGTGGTA131050.018.9855024
GTGGTAT137500.018.094915
GCAGTGG139950.017.9029982
CAGAGTA141700.017.455517
ACGCAGA141500.017.35838114
AACGCAG143050.017.21800613
AGCAGTG145200.017.0214021
CGATCTA32950.016.9603488
TCAACGC145400.016.75007411
TCTTCCG114250.016.672733
Produced by FastQC (version 0.11.2)