FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n01_rnaseq_dmso1_10_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n01_rnaseq_dmso1_10_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14768828
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158500.10732063505648519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG37650.022.72251322
GAGTACG57650.022.55926319
TCCGATC83400.022.4259786
AGTACGG59050.022.1412520
GTACGGG59400.022.12626621
AGAGTAC92500.021.0338518
CCGATCT92500.019.9894817
CGCAGAG98850.019.71769315
CTTCCGA99550.018.7878114
CGATCTA32950.018.5306428
AGTGGTA109450.018.4126054
GTGGTAT114750.017.6523915
ACGCAGA111350.017.59715514
GCAGTGG117900.017.1239072
TCTTCCG110650.017.1213933
CAGAGTA115800.017.09955217
AACGCAG118050.016.59791213
AGCAGTG122300.016.3426251
TCAACGC120550.016.28237211
TTCCGAT118850.015.8530065
Produced by FastQC (version 0.11.2)