FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n01_rnaseq_dmso1_10_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n01_rnaseq_dmso1_10_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15343336
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161700.10538777225500373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC93700.022.7600296
TATGCCG25400.021.73136749
CCGATCT103100.020.744157
CTTCCGA108150.020.1337834
GAGTACG59950.020.08201619
TACGGGG39450.020.02394522
CGATCTA34200.019.8698318
AGTACGG61350.019.68003820
GTACGGG62500.019.3724921
AGAGTAC94500.017.92329818
TCTTCCG122150.017.7414913
GTATGCC31700.017.52077348
GAGTACT52150.017.39698220
TTCCGAT127100.017.1862895
CGCAGAG102550.016.61726615
CCGCTAC34600.016.2533734
GCTCTTC141900.015.6430361
AGTGGTA111150.015.5232664
ACGCAGA111450.015.44502914
CGTCTGA39150.014.80286517
Produced by FastQC (version 0.11.2)