FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n01_rnaseq_bpa2_10_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n01_rnaseq_bpa2_10_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14702536
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159950.10879075555400783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC110850.027.26197818
GAGTACG63950.027.18829219
TACGGGG45950.026.125722
AGTACGG67250.025.8030220
CGCAGAG118900.025.5612215
GTACGGG70000.024.6895921
AGTGGTA126350.024.3064734
ACGCAGA130300.023.53666114
GTGGTAT133800.022.927375
TCCGATC75100.022.8822146
CAGAGTA133650.022.79191217
GCAGTGG137350.022.4620822
AACGCAG137350.022.35268213
TCAACGC137100.022.3181111
AGCAGTG138650.022.1063461
TGGTATC148650.020.6137356
GGTATCA150200.020.5327077
GCAGAGT150550.020.34790616
ATCAACG156550.019.67755310
CCGATCT87200.019.6615857
Produced by FastQC (version 0.11.2)