FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n01_rnaseq_bpa2_10_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n01_rnaseq_bpa2_10_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14590793
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158800.1088357569050565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG39800.025.568722
GAGTACG57750.025.32777219
AGTACGG59900.024.59145420
AGAGTAC100050.023.6875318
GTACGGG62100.023.49732821
CGCAGAG105750.022.5739415
TCCGATC62850.021.853316
AGTGGTA115600.021.1654644
ACGCAGA117600.020.38727214
CAGAGTA121200.019.83856617
AGCAGTG123600.019.8307251
GTGGTAT123950.019.7117945
GCAGTGG125050.019.6784732
TCAACGC126250.019.0992811
CCGATCT73850.018.96487
AACGCAG128200.018.75482413
TGGTATC137900.017.6676946
CTTCCGA80900.017.4467374
GGTATCA140600.017.2972287
GTATCAA143200.017.1035048
Produced by FastQC (version 0.11.2)