FastQCFastQC Report
Thu 2 Nov 2017
HMVKFAFXX_n01_rnaseq_bpa1_10_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVKFAFXX_n01_rnaseq_bpa1_10_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17622163
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193650.10989002882336293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC95550.024.0893866
CCGATCT104850.021.846467
GAGTACG67550.021.65325419
AGTACGG68100.021.4277820
TACGGGG45750.021.41405122
CTTCCGA110200.021.1685924
AGAGTAC105250.020.6490418
GTACGGG71850.020.35685321
CGATCTA38450.019.6482688
CGCAGAG112150.019.3171515
TCTTCCG129600.018.0530413
TTCCGAT137800.017.1292155
ACGCAGA128600.017.08760314
CAGAGTA130900.016.91908517
AGTGGTA131900.016.848764
GTGGTAT137000.016.1713035
AACGCAG138750.015.98626913
GCAGTGG141700.015.9280232
TCAACGC140750.015.71031611
GCTCTTC154400.015.2913361
Produced by FastQC (version 0.11.2)