FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n02_dmso_2_10_18_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n02_dmso_2_10_18_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27266140
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG555880.20387190852830653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA351500.016.78720369
AGTGTAG505700.015.82153669
GAGTGTA600550.013.42582568
GACGAGT1019550.012.80635969
ACGAGTG841350.012.75605969
GTAGATC199400.012.3661569
CGACGAG1278600.012.30209369
CCGACGA1518400.012.289803569
GCCGACG1864200.012.16345269
TGTAGAT321900.011.91344769
CGAGTGT687600.011.88683169
AACCGCG64500.011.8782627
TCTCGGT72600.011.63812669
CTGCCGC129550.011.52667769
TATACCG93600.011.1751055
GCGGTAG49050.010.83224811
TAGACCG59900.010.7194125
TGCCGAC2207550.010.67310969
ATCTCGG102250.010.35448969
ACCGCGG63400.09.7972598
Produced by FastQC (version 0.11.2)