FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n02_dmso_1_10_25_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n02_dmso_1_10_25_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25875904
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580100.22418540430510178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA319900.016.149569
AGTGTAG448550.014.49312269
GACGAGT915100.013.21310469
ACGAGTG755550.012.85834669
CCGACGA1362250.012.47346969
GAGTGTA530700.012.438107569
CGAGTGT613200.012.34507969
CGGGACT35750.012.25661410
CGACGAG1144250.012.23676569
GCCGACG1661500.012.0991669
TCTCGGT68450.011.84011469
GTAGATC191450.011.51083969
ATCTCGG89450.011.45084769
CTCGGTG58550.011.19149769
AACCGCG62600.011.1357597
TGCCGAC1987950.011.01441269
TGTAGAT312450.010.77285469
TCGGGAC41700.010.5911849
CTGCCGC100900.010.49321869
ACCGCGG59950.010.4182418
Produced by FastQC (version 0.11.2)