FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n02_dmso_1_10_18_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n02_dmso_1_10_18_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28695646
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG553180.19277488996065814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA403050.016.94239469
AGTGTAG557700.015.43520669
GAGTGTA682300.013.97617469
TCTCGGT83300.013.70413169
CGAGTGT807350.013.39623469
CGGGACT49200.013.18357410
CTCGGTG65650.012.87063169
ACGAGTG991250.012.66793969
GACGAGT1195650.012.64258769
TCGGGAC53800.012.3777569
ATCTCGG121750.012.32221569
GTAGATC228350.012.12782869
CGACGAG1474350.011.78490569
CCGACGA1735250.011.68449469
GCCGACG2098100.011.65107669
TATACCG96850.011.5484285
CTGCCGC121250.011.5481669
TGTAGAT368800.011.18429969
TAGACCG57800.011.1684075
TGCCGAC2490350.010.38927369
Produced by FastQC (version 0.11.2)