FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n02_bpa_2_10_25_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n02_bpa_2_10_25_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22079793
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321280.14550861052003522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT99250.017.92965169
CTCGGTG76450.017.45766369
ATCTCGG135600.015.89545469
GTGTAGA474000.015.76642569
TCGGTGG53650.015.74885669
AGTGTAG635700.015.67827569
CTGCCGC123000.013.149837569
ACGAGTG1155000.013.11990569
GAGTGTA784750.013.1179469
GACGAGT1406850.013.05587669
CGAGTGT934550.012.78285869
GATCTCG167700.012.64700868
GTAGATC284700.012.34353169
CCGACGA2065150.012.08536169
TAGACCG50800.011.8916565
GCCGACG2507700.011.883409569
CGACGAG1724700.011.80952769
TGTAGAT400150.011.41084569
AGATCTC189600.011.258903567
AACCGCG48550.010.9467777
Produced by FastQC (version 0.11.2)