FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n02_bpa_1_10_25_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n02_bpa_1_10_25_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24203802
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312020.12891363100722772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG91450.022.85304569
TCTCGGT127750.021.97448269
TCGGTGG58600.020.4214769
AGTGTAG916200.017.04775469
GTGTAGA660500.017.0476969
ATCTCGG189250.015.9268869
GAGTGTA1164150.015.60897569
CCTTTGG70200.014.5825311
CGAGTGT1453350.014.58165569
CTGCCGC169050.014.1579269
AACCGCG42150.014.0014587
ACGAGTG1815700.013.87114669
TATACCG63400.013.4494535
TGTAGAT540050.013.41674769
TAGACCG49300.013.3046545
GTATTAG117500.013.0978641
GATCTCG230050.013.01206868
GACGAGT2219250.012.832870569
AGACCGT53450.012.786316
ACCGCGG42500.012.3420978
Produced by FastQC (version 0.11.2)