FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n02_bpa_1_10_18_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n02_bpa_1_10_18_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27646088
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313660.11345547333857868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA464550.016.07279469
AGTGTAG637900.015.53817869
TCTCGGT89550.014.40362969
ATCTCGG123100.014.28821869
GAGTGTA775500.013.03466869
GTAGATC266400.012.8682169
CTCGGTG76150.012.68101769
ACGAGTG1103150.012.56462169
TGTAGAT407750.012.33188669
GACGAGT1324200.012.16788869
CGAGTGT903350.011.98016669
CGACGAG1629100.011.36398769
CCGACGA1894850.011.18986869
GATCTCG155750.011.18219168
GCCGACG2262950.010.8129369
CTGCCGC152900.010.66889969
TATACCG98150.010.4107785
AGATCTC172450.010.21932167
GTATTAG146600.010.1672151
CTATACT134550.09.8559314
Produced by FastQC (version 0.11.2)