FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n01_dmso_2_10_18_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n01_dmso_2_10_18_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27266140
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGA327450.016.71071669
ACTCCTG511400.015.415668569
TCCTGAG240450.014.89393469
GACTCCT533100.014.64546668
CCTGAGC167150.014.53125669
CACGAGC93250.013.91156169
GAGACTC792650.013.2364669
CGAGACT985550.012.80215769
ACGAGAC1271100.012.42061869
AGACTCC642200.012.40482169
GAGCCCC53450.012.39339869
CCACGAG1878900.012.31944269
CACGAGA1523800.011.82575769
CTGAGCA150000.011.52347269
TAGACCG60450.011.0165125
AACCGCG66000.010.8188337
CCCACGA2229150.010.605103569
AGACCGT66600.010.361836
TACACTG152500.010.1592025
TATACCG92150.010.0351195
Produced by FastQC (version 0.11.2)