FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n01_dmso_1_10_25_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n01_dmso_1_10_25_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25875904
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCA295850.018.11073569
ACTAGGC384700.017.35381569
TAGGCAT233300.014.418769
GAGACTA751500.013.30930769
GACTAGG507550.013.30295769
CGAGACT928800.013.24260869
GAGCCCC30750.012.79074769
AGACTAG613600.012.57252869
ACGAGAC1160050.012.37831769
CACGAGA1399400.012.21132169
CCACGAG1697350.012.16953769
CGGGACT39700.011.72975110
AGGCATG202300.011.56298569
GGCATGA158100.011.50045369
CCCACGA2017900.010.96643569
TCGGGAC44250.010.9134489
CTGGACG45350.010.7276775
GGACGAC49800.010.6668067
GTCTTAG45100.010.6361281
GCGGTAG49200.010.58662111
Produced by FastQC (version 0.11.2)