FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n01_dmso_1_10_18_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n01_dmso_1_10_18_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28695646
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT361000.018.52193869
ACCGTAC491200.017.243869
GACCGTA619500.015.27094769
CGTACTA287250.014.25707869
CACGAGC107200.014.19330969
GTACTAG223400.013.25085769
GAGACCG972600.013.09331669
AGACCGT811800.013.05180669
CGAGACC1213150.012.73246469
CGGGACT53850.012.17073810
ACGAGAC1476100.011.85271169
CCACGAG2148350.011.73475269
TCGGGAC56900.011.6397219
GAGCCCC45650.011.639111569
CACGAGA1751200.011.14132169
CTAGATC108450.011.07085768
AGATCTC86750.010.97689469
TATACCG96950.010.8196145
CTGGACG44600.010.75395
AACCGCG64950.010.3031877
Produced by FastQC (version 0.11.2)