FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n01_bpa_2_10_25_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n01_bpa_2_10_25_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22079793
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC45500.017.2126869
ACCGAGG598800.016.9048969
CCGAGGC458250.016.59369769
CACGAGC89900.016.50223769
GACCGAG757000.013.691092569
GAGACCG1147300.013.34879969
CGAGACC1440000.013.00503169
TGATCTC89100.012.6233369
AGACCGA917050.012.53938969
CGAGGCT348750.012.50451769
GCTGATC117950.012.22656768
TATACCG74750.012.0018265
CCACGAG2559700.011.93395769
ACGAGAC1743050.011.87416569
AGGCTGA206550.011.69248769
CACGAGA2086900.011.63375669
GAGGCTG336050.011.06748369
AGACCGC41300.011.02701469
AGACCGT54850.010.6943776
CGGGACT33400.010.43100910
Produced by FastQC (version 0.11.2)