FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n01_bpa_1_10_25_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n01_bpa_1_10_25_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24203802
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG73550.022.93854569
CACGAGC97650.017.80729769
ACCAGAG907150.017.55603269
CCAGAGA655000.017.27188769
AGGATCG119200.016.7877169
GAGCCCC44600.016.63195469
GACCAGA1161000.015.90760169
TAGACCG50700.015.7891675
TATACCG63550.015.0941415
CAGAGAG495300.014.92075669
GATCGGG66700.014.84553169
AGACCAG1465450.014.47446669
CCTTTGG74600.014.4341731
AACCGCG43250.014.1964967
AGACCGT55700.014.0000556
GAGACCA1849350.013.96221669
GAGGATG60050.013.50188969
GAGGATC163950.013.17294567
ATACTGA90400.013.1682226
CGAGACC2270400.012.80742569
Produced by FastQC (version 0.11.2)