FastQCFastQC Report
Thu 2 Nov 2017
HMVK3AFXX_n01_bpa_1_10_18_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK3AFXX_n01_bpa_1_10_18_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27646088
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAG467950.016.37527769
ACAGGCA629400.016.00647569
AGGCAGA354900.013.96008369
GACAGGC749750.013.78224369
GAGACAG1149250.012.491734569
GGCAGAA299750.012.25829269
CGAGACA1369400.012.14888569
AGACAGG914650.012.05189969
GAGCCCC47350.012.02274169
AACCGCG73850.011.7238037
ACGAGAC1648550.011.48135769
TAGACCG67250.011.3904755
CACGAGA1950600.011.06011869
CCACGAG2309750.010.92669869
TATACCG101600.010.8676965
GTATAGG106900.010.3631891
ACCGCGG60800.010.2121918
GCGGTAG55950.010.11095511
ATACCGT88200.010.0932076
GCAGAAA303350.010.04283369
Produced by FastQC (version 0.11.2)