FastQCFastQC Report
Sat 22 Jul 2017
HMVK2AFXX_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK2AFXX_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119074537
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCAT1721385014.456365259686041No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC1309438110.99679354621383No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGCATCCGCCGTGGGCGGT1245311910.458255235542087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74769096.279183768734704No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAAACCACACCGTGGTAATC48587154.080397977948888No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGGAAGG38127303.2019692001825715No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA32797052.7543294163722005No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT30892392.5943741439868035No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAGACCACACCGTGGTAATC29940302.514416663236742No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC17349231.4570058752359456No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTCGGCCGC16394031.3767872135417163No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCTACCTTAGACGGC13877911.1654809121785625No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGACGGC10993530.9232477637095494No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATAAAGGCG8398930.7053506326041814No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAA7670530.6441788642016723No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGTCCCGCCTTAGGCGGC7643070.6418727456399852No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAACGCG7612470.6393029267038007No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGCCCTGCCTTAGACGGC7532850.6326163586090618No Hit
GGATACCGTGTTACGACTTCTTCATCCTTTAAGACATGCAACTAAATCAA7075920.5942429152590365No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC5492340.4612522658811598No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGGCGGC4805210.40354639380206025No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGAAAGG4340830.3645472919201861No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCCACCTTAGACGGC4330140.3636495349127412No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGGGGAT3494760.2934934779549048No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC3060950.2570616755788855No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGATTTACACAA3042500.25551222592618605No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG2994990.25152228809422117No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC2918060.2450616289190358No Hit
AGTAACCTTGTTACGACTTGTACTTTCTCTAAATGTCCAAACTTGCACAA2784780.2338686397747656No Hit
GGCTACCTTGTTACGACTTCACCCTCCTTAAAAAACCTAGATTCGAATAT2755440.231404636912424No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTAGGCCGC2752240.23113589767726747No Hit
GGAAACCGTGTTACGACTTTTGCATCCTTTAGAGGGCTCTATTTACGCAA2639840.22169643204239375No Hit
AGATACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTGAACTG2559960.21498802888479845No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGAACAC2424440.20360692227591867No Hit
GGTTACCTTGTTACGACTTAACCCCAGTCGCTGCACCCACCGTAGACGGT2181400.1831961773657789No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTAGGTCGC2015020.16922341675785815No Hit
AGATACCTTGTTACGACTTAACCCTCCTTGCTAAATCTCAGTTCGACAAC2001810.16811402760272753No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA1928330.1619431029154453No Hit
GAAAACCTTGTTACGACTTCTTCTTTTTCTAAGGGTTGAGGTTTACAAAA1905820.16005269035814096No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA1873650.15735102123470782No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTTGAACAC1833530.1539817030739326No Hit
GGATACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA1644740.13812692800980617No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG1609930.13520354901736883No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCCAGGCGCGGGGGCTCGCGGCC1550140.13018232437049074No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG1522550.12786528827737537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA89290400.093.9467857
TGTTACG90654050.093.9451759
CCTTGTT89405650.093.944556
ACCTTGT89366550.093.9365
TTGTTAC89281350.093.8145458
AAACCTT35326150.093.8144763
TACCTTG53843700.093.619674
ACCGTGT1370800.093.580375
TCTCGAT4008450.093.4741294
AACCTTG35915500.093.380564
GGCTACC13016150.093.284411
GTTACCT38555750.093.266012
GAAACCT30818150.093.173162
CTCATGG6320600.093.11484594
GCCTTGT188500.092.992195
GATACCT2148800.092.8609852
CGTGTTA1376950.092.848047
GGAAACC31163200.092.712631
ACCTCGT196200.092.551415
CCGTGTT1401800.092.524386
Produced by FastQC (version 0.11.2)