FastQCFastQC Report
Sat 22 Jul 2017
HMVK2AFXX_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK2AFXX_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119074537
Sequences flagged as poor quality0
Sequence length12
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGG2626221422.055272824617408No Hit
ATGACTCATTCG23627851.9842907304355086No Hit
GGAGTCGGTCTA14809091.243682349988898No Hit
TTGAAGTCCATC12125961.0183503799809024No Hit
CCACTAATTGAC11469380.9632101277874379No Hit
AGATTCGTTGTC11247050.9445386296148269No Hit
TCTCACTAGGTA10657940.8950645762326164No Hit
GCAATAGCTGCT10517080.8832350110250692No Hit
ACTCTTCTAGAG10336400.8680613219600425No Hit
AATTTGATCCCG10279300.8632660062327179No Hit
TGGCTCTACAGA10208070.857284038820155No Hit
GGTTTCAAGCTT10198110.8564475879507304No Hit
CTGCATAGATCT10073420.8459759956908336No Hit
ATAAGGCCTTCT9931780.834080925294717No Hit
GACAATCTGCTT9927220.8336979718846189No Hit
TTCAACAGGTGC9838240.826225341527047No Hit
GACTTTCCCTCG9779620.821302374663023No Hit
TGAACTGCCGTA9711400.8155731900935295No Hit
CTTGCCTTGTGC9669910.8120888179477028No Hit
AACGGATAAACG9597550.8060119519927254No Hit
TCGAGACGCTTA9596610.805933009842398No Hit
AGGAGTAACACA9531650.8004776033687202No Hit
CGACTCCAAGAT9492210.7971653922954158No Hit
GGTAATCCTGTG9117470.7656943482383644No Hit
GTTGACGACAGC9072910.7619521543888094No Hit
GACAGGAGATAG8890820.746660052098292No Hit
AGAGTCCTGAGC8795960.73869361339612No Hit
TCTATCGGGTTA8675490.728576421002586No Hit
CTCAGTATGCAG8653610.7267389164822031No Hit
CTAGCGAACATC8588030.7212314417817136No Hit
GCTGATGAGCTG8484650.7125494848659374No Hit
TGCCTACAAGCA8469600.7112855706505917No Hit
GCGGCAATTACG8304850.6974496990905789No Hit
TCCGACACAATT8226090.6908353546652883No Hit
CTAGAGACTCTT8185170.687398851695724No Hit
ATCCCGAATTTG8063630.6771917996204344No Hit
TACACGATCTAC7741970.6501784676265422No Hit
ACGGGACATGCT7735380.6496250327641417No Hit
GACGGAACCCAT7687500.6456040219581118No Hit
CTTATCAAGGCG7612020.6392651352488568No Hit
CCTTATAGGCGA7463590.6267998337881423No Hit
ACAAAGGCCCTG7450870.6257315953283951No Hit
ACGCGCAGATAC7297440.6128463888127484No Hit
TCACAGATCCGA7165480.6017642546029803No Hit
GAACTAGTCACC7125410.5983991354927544No Hit
TGCTCGTAGGAT7098620.5961492842084282No Hit
GGATCGCAGATC7093540.5957226606726173No Hit
ATTCCTGTGAGT7091300.5955345432080076No Hit
AAGAGATGTCGA7038670.5911146225997923No Hit
GATAGTGCCACT7037660.591029801778696No Hit
TCCGAATTCACA6962400.5847093908918579No Hit
TTACTGTGCGAT6952260.5838578234404557No Hit
AAGACCGGTGCT6891580.5787618556937996No Hit
CTAACCTCCGCT6856360.5758040444868578No Hit
GAAGTTGGAAGT6821830.5729041801774968No Hit
GGAGACAAGGGA6818410.5726169651199231No Hit
CGATGTCGTCAA6806780.5716402659621511No Hit
CCTCGTTCGACT6686840.5615675835044398No Hit
ATGGCAGCTCTA6680720.5610536197172029No Hit
ATGCACTGGCGA6614500.555492397169682No Hit
CAACCTTTACAG6570720.5518157085086965No Hit
AGTAGAGGGATG6561450.5510372045368525No Hit
CCACGAAGCTAT6521770.5477048380209113No Hit
TAGGAACTGGCC6482160.5443783501757391No Hit
GCCTCCCTTATG6379150.5357274662340279No Hit
CACGCCATAATG6368170.534805354733397No Hit
CCTACGCCTCTA6366510.5346659462551595No Hit
ACCTAGAGTCGG6159660.5172944741326183No Hit
CTATTTGCGACA6113450.5134137116149358No Hit
CATAGTTTAGCC6106520.5128317232087999No Hit
TCTAGCGTAGTG6013720.50503828538926No Hit
GTCTAATTCCGA5849380.49123684604375156No Hit
TGAGAACCTCCA5742520.4822626352097426No Hit
TAATGACCACGC5729750.48119019769944604No Hit
AAGGAGCGCCTT5646000.47415678802933325No Hit
CTACTGATATCG5550920.46617187350474437No Hit
GAGGCTCATCAT5482020.4603855818477799No Hit
AATGCCTCAACT5467570.4591720562390262No Hit
CATCAAACCTCA5357560.44993330522041No Hit
ACGCATTGCATT5335260.4480605286754128No Hit
CTTCGGCAGAAT5279860.44340798066676507No Hit
GCACCCAAAGTG5241420.44017975060444703No Hit
ACCTGTCTCTCT5171890.4343405509105612No Hit
AATCCGTACAGC5055750.4245869962945983No Hit
CAGGGTGATACC5016790.4213150961065673No Hit
AATCAGTCTCGT5016450.4212865425628319No Hit
GGCCACGTAGTA4902220.411693391677853No Hit
CGGAGCTATGGT4878990.40974251279263846No Hit
CTTGTGTCGATA4858470.4080192224471971No Hit
TTGCCACTAGAT4828820.4055291854714497No Hit
CAGTAACGGCCA4775140.40102108480169857No Hit
ATGTGCACGACT4756840.39948423230064717No Hit
GTCAATTGACCG4535290.3808782393166055No Hit
ATCGAATGCGGC4133360.3471237515708333No Hit
GTATGCGCTGTA4132920.3470867999259993No Hit
CGTGTTTCAAAC4113620.3454659664139614No Hit
TGAGCCGGAATC4025170.3380378459922124No Hit
ACACGTAAGCCT3994580.3354688668661378No Hit
ATCGATCTGTGG3902100.32770230297011355No Hit
GGTGTCTATTGT3802470.3193352748455365No Hit
GGAAACCACCAC3796400.31882551010884885No Hit
GAGTGGTAGAGA3726150.31292584408705276No Hit
CAGGCGTATTGG3589730.30146915456828527No Hit
ACCGTGCATAGG3566910.29955270789757515No Hit
CCAGTGTATGCA3518360.29547542981418434No Hit
GACTTGGTATTC3412330.28657092321929417No Hit
CAAGCATGCCTA3378960.28376847688267726No Hit
AGCTGTTGTTTG3344860.2809047244080403No Hit
TCCTCTGTCGAC3254490.27331536044519744No Hit
NNNNNNNNNNNN3165760.2658637253403723No Hit
TTCAGACTACCA3163400.26566553015444433No Hit
TCCCATTGAAGG3161720.2655244420559872No Hit
TAGGATTGCTCG3133490.2631536581158405No Hit
TGCTCAGTATGT3123000.26227269731059294No Hit
GCGCTAAAGTTC3111790.2613312701774352No Hit
TCCAAAGTGTTC3101200.260441911271089No Hit
TATCGTTGACCA3085610.2591326473098106No Hit
GCACACACGTTA3008290.2526392355403406No Hit
CATTCGATGACT2918620.2451086582851882No Hit
ACACCTGGTGAT2894450.2430788372496464No Hit
CCTTTCTTCTCA2840110.2385153091126443No Hit
AATGGCCTCTAT2787090.23406263591014423No Hit
CCTGTATCCCGT2663970.2237228938374961No Hit
GAATCTTCGAGC2635060.22129500280987868No Hit
GGCTCTGCTCTT2632150.22105061806790816No Hit
ATTCTAGCGTTG2590090.2175183767458193No Hit
TTCCTAGGTGAG2564450.21536510362412747No Hit
GAGTCAACCGCA2402210.2017400244016905No Hit
CCTGAACTAGTT2325620.1953079187702405No Hit
TTGCGCATACTA2267160.19039838886797433No Hit
ACGTGTACCCAA2178390.18294339452270977No Hit
ACCACCAGAACG2171850.18239415871085857No Hit
ATGAGACTCCAC2138150.17956399864061617No Hit
TCGAGGACTGCA2131020.178965214032283No Hit
TGCCAGGCTAGA2120960.1781203650617596No Hit
GCGGATGTGACT2103170.17662634287631118No Hit
GCACGACAACAC2090590.175569861758102No Hit
ATTTCGACATGC2052200.17234583074633328No Hit
AGGCTGATTTGC2028880.17038739357012994No Hit
TACAGATGGCTC2023440.1699305368703638No Hit
AGGCTACACGAC2015160.16923517409939626No Hit
GTCATATCGTAC1942510.16313395365123276No Hit
ACGTTAGCACAC1847050.15511712634246902No Hit
GGATGGTGTTGC1619070.13597113545778472No Hit
ACGCCACGAATG1296240.10885954568103842No Hit
TGCCCATTTAAG1294430.108707540051153No Hit
AGAGGAGACCAA1268690.10654586882836252No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCC751.7497079E-46.00051985
CTCAAAG1303.6416168E-96.00051934
TCCTGGC550.009153736.00051935
TTCGTTA1701.8189894E-126.00051934
TTTCCGT550.009153736.00051934
CTTCTTG550.009153736.00051934
CAGCTTG2550.06.00051935
TAGTGAG550.009153736.00051935
TCAAGGG1101.8131868E-76.00051935
GTCGGAC1351.373337E-96.00051934
TGTATGA704.6931492E-46.0005195
GTAGGTC1900.06.0005194
CTGCAGT704.6931492E-46.0005195
ACGCGAG600.00339774145.9998971
TTACTGG550.0091635355.9998971
GCCTTCG1101.8170431E-75.9998676
TATGGAG909.158786E-65.9998676
TGTGAGG550.0091640145.9998676
TGTGACA1351.376975E-95.9998676
GCTTCTT4600.05.9998676
Produced by FastQC (version 0.11.2)