FastQCFastQC Report
Sat 22 Jul 2017
HMVK2AFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVK2AFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119074537
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGG1709601614.357407075200301No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG1281148810.759217144804015No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC111140269.333671396093692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74701346.2734940552403735No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATATAAATAATT38749503.2542221852183224No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGG30001292.519538665096804No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG29639372.4891442576005987No Hit
ACACCATGGGAGCGGATTGCACCAGAAGTAGTTAGCTTAACCGAAAGGAG28379802.383364295592432No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGCAAAGG15658181.3149897866073583No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTGAGCTAACCGCAAGGAG14677151.2326018953993498No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCCGCAAGGG14634411.2290125469897901No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGAAAGGG12758481.0714700490500333No Hit
ACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGG12676891.0646180383636512No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG11505610.9662527598154759No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTGGAG10197760.8564181945968851No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGAATGCCAAACTGGCTACC9324230.7830582620699168No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCGAA8018430.6733958579238482No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCTCA7564110.6352416050124974No Hit
ACACCATGGGAGTTTGTAACGCCCAAAGTCGGTGGCCTAACCTTTATGGA7435490.6244399673794239No Hit
GCTTCAACTGATGAGATCGTACAACAATAACAAATCTGTAAGTCAGGTTT7160860.6013762623322231No Hit
ACACCATGGAAGTCTGCAATGCCCGAAGCCGGTGGCCTAACCACTTATGT5186590.43557507177206156No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGTAAGGAG4939780.41484771845050294No Hit
ACACCATGAGAGTTTGTAACACCCAAAGTCGGTGGGGTAACCTTTTGGAG4473370.3756781351163263No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATACAAAAAATA4402860.3697566340316738No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATGCGCTTAGCCTG4316160.36247548037915106No Hit
ACACCATGGGAGTTGATTTCACTCGAAGTGGAAATACTAAACTAGTTATC3516020.29527891424847613No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG3485970.2927552848683342No Hit
GCACCTACCGATTGAATGGTCCGATGAACACTTTGGATTTTGATATATTG3015300.2532279424273554No Hit
GCTGTCCGGGACTGAGCTGTTTCGAGAAGACTGAGGACCGCTTTTGTGAG2993770.2514198312608178No Hit
TCACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG2866520.2407332476127957No Hit
GTTACTATCGATGAATGTTCAATTGAGTTCTTAGGACGGCCACTACGGTT2830050.23767046014212093No Hit
ACACCACCCAAAAAGGGCTTGGATGAGGATGTAATGTTTTTTGTTATATT2745350.23055726851157105No Hit
GCTGTCCGGGACTGAGCTGTCTCGAGAGGACTGCGGACTGCTGTATCGAG2527140.2122317721042241No Hit
AAACCATCCGAGTTGGGTTCCAGTGAGGCTGCCTCTAATTAGGGTTGTCG2436760.20464156833127134No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTCACCTCTAATTAGGGTGTTCG2416350.2029275158970385No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTAGGA2364960.19861173174244634No Hit
GCTGCAACCGATCGGAGGGTCCTGTGAATTCATCGGATTGACCATCTTCG2187830.18373617526642158No Hit
AAACCATGGGAGTTGTATTCGCCTTAAGCCGGGATGCTAAATTGGCTACC2181780.18322809014995373No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGCAAGGG2159040.1813183619601225No Hit
GCTTCTATTGATTGAGTGTTGTGGTGAATTATTCGGATGAATTCGATAGT1966400.16514026000369836No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGGG1929550.16204555974884874No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCTTAGGAGG1913980.16073797540779017No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCCTCGGGAG1910570.16045160016032647No Hit
AAACCACCCGAGTTGGGTTTCAGTGAGGCTGCCTCTTTTTGGGGTTGTCG1852120.15554291006817017No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTTGCCTCTGATTAGGGTGTTCA1827900.15350888998207904No Hit
ACACCATGAGAGTTGGCAATACCCAACGTCCGTGAGCTAACCGCAAGGAG1774840.14905285753913955No Hit
GCTACTACCGATTGGATGGCTTAGTGAGGCCCTCGGATCGGCCCCGCCGG1661210.13951009526075253No Hit
ACACCACGAGAGTTGGTTGCACCTGAAGTAGCAGGCCTAACCGCAAGGAG1484370.12465889327791381No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGAGGTAACCTTTTAGGA1440330.12096036955407184No Hit
TCACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC1396310.11726352545044959No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCAGCTTAGAGAA1379080.11581653263115353No Hit
GCACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG1345900.1130300426866241No Hit
GCTCCTACCGACTGGGCGCGGCGGCGAGCGCCCCGGACGCGCGAAGGGCC1335280.11213816434994829No Hit
ACACCACGAAAGTCGGTTTTACCCGAAGCCGGCGAGCCAACCAGCAATGG1211920.1017782668346634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC23097450.093.870552
TACTACC23174300.093.860153
ACTACCG23168100.093.84974
CCGATTG28916350.093.8482748
CATGGGA40963700.093.8418665
ACCATGG41592300.093.838063
TGGGAGT31953950.093.8256157
CCATGGG41045600.093.821754
CGGAACT4646200.093.81827
ATGGGAG40990400.093.815526
CATGAGA8982150.093.813655
CTCCTAC4328950.093.80252
CGATTGA25168500.093.78889
GGGAGTG15534550.093.7702268
ATGAGAG8994700.093.7695856
CCATGAG9010200.093.76364
CCGGAAC4655850.093.7420966
TGAGAGC2042400.093.6936657
CACCATG50378050.093.677662
CCCGGAA4656450.093.633045
Produced by FastQC (version 0.11.2)