FastQCFastQC Report
Sat 8 Apr 2023
HMVCVBGXN_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVCVBGXN_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508849031
Sequences flagged as poor quality0
Sequence length10
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGGTGTC48498840895.31086401931263No Hit
AGATCTCGGT85174461.6738650328686586Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG18091060.3555290252680073No Hit
GTTGGTGTCG9522970.18714725625565748No Hit
GTTTGGTGTA8733150.17162556019488617No Hit
GTTTGGTGTG8670750.1703992632738255No Hit
GTTTGTGTCG6954760.13667629446659985No Hit
GTTTGGGGTC5797310.11392986223452198No Hit
GTTTGGTGGC5378260.10569461023499521No Hit
GGTTGGTGTC5164280.10148943370985805No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)