FastQCFastQC Report
Sat 8 Apr 2023
HMVCVBGXN_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMVCVBGXN_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508849031
Sequences flagged as poor quality0
Sequence length10
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCT47525069093.39718876265286No Hit
GGGGGGGGGG125431942.4650128497542525No Hit
CCAGCTTCTA57773461.1353752582855954No Hit
CCCAGCTTTA11046830.21709444898205965No Hit
CCCAGCTCTA10818190.2126011712892502No Hit
CCCGCTTCTA7578850.14894103237468875No Hit
CCCAGCTTCA7478770.14697424077437224No Hit
CCCAGTTCTA6999710.13755966059803698No Hit
CCCACTTCTA6975280.13707955749256404No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)