FastQCFastQC Report
Mon 23 Oct 2017
HMTYCBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTYCBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307334218
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14820880.48223982661117154No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT13020470.4236583249574898No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC4984020.16216938134757256No Hit
GTACTGGACCATGTGGAAGCTGCCCATGTTCGGGTGCACCGACGCCACCC4738950.1541953262099829No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG4570960.14872928988336728No Hit
TGCCAGCTCTGACCGAAATCTTTGGAGATGATTCTGTATTGCAATTTGGT3991480.12987424654419705No Hit
GTATTCATGTTTGGCATATGCCAGCTCTGACCGAAATCTTTGGAGATGAT3438880.11189382107787293No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG3202130.10419048099616424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT2947250.021.3994436
GTTATAC3510400.021.339063
TATACCG3636500.020.9055845
TTATACC3591550.020.7404564
TACCGGT3578100.020.6691067
GGGGTAT3479700.020.42595122
ATACCGG3691200.020.4081696
TGTTATA3781450.020.2583222
GGTATTC3522000.020.22671924
GGGGGTA3564700.020.2083121
GGGTATT3574650.019.92840623
TGGACCA3367000.019.7335955
TGGCATA3431600.019.72134236
TCAGGGG3682700.019.68390318
GGCTTCA3685250.019.6622514
GTGTTAT3953350.019.4094811
TGGCTTC3770500.019.16257713
GCTTCAG3810750.019.13577315
TTCAGGG3816750.019.07238217
GTACTGG2635450.019.0277561
Produced by FastQC (version 0.11.2)