FastQCFastQC Report
Mon 23 Oct 2017
HMTYCBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTYCBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307334218
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG3465050.11274533706494082No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACCG5464650.013.9100951
CCTACAA5975200.013.9089761
CGCATCC8438450.013.8979345
TCGCATC8448450.013.8908524
AACCGAT5491550.013.8783273
CCACACT6270550.013.8661862
TACAAGA5982300.013.8645253
TCAGAAC4768150.013.8605512
CAACCGA5497300.013.8578512
AACTACC4760550.013.8529886
TACTGCA5818150.013.8472431
GTCGCAT8479650.013.8410933
TTGTCGC8467400.013.8401191
CCTCTCT3190650.013.8313184
TACACCT2446850.013.8280541
TTCCACC2585200.013.8181682
CGCATCT3609650.013.80639655
CTACAAG6014800.013.8057292
AAGATGC5991850.013.7964046
AGAACGA5621350.013.7952652
Produced by FastQC (version 0.11.2)